Journal: bioRxiv

Article Title: Comparison of immunohistochemistry methods in embryonic chicken corneal tissue

doi: 10.64898/2026.03.30.715369

Figure Lengend Snippet: (A–C) Limbal region, showing TAP1 signal primarily associated with the stromal compartment, with marked differences in signal intensity and continuity across processing methods. (D–F) Central cornea, showing background or negative staining across all processing methods. (G–I) Kidney tissue, serving as a TAP1-positive control, showing robust TAP1 expression with clear cellular and subcellular definition in Wax and Wax AR sections and reduced structural resolution in cryo-embedded tissue. * marks one positive cell in stroma

Article Snippet: The monoclonal antibody TAP1 from DSHB binds chicken class I MHC antigens primarily found on B cells, macrophages, and dendritic cells.

Techniques: Negative Staining, Positive Control, Expressing

Journal: bioRxiv

Article Title: Comparison of immunohistochemistry methods in embryonic chicken corneal tissue

doi: 10.64898/2026.03.30.715369

Figure Lengend Snippet: (A–C) Limbal region, showing TAP1 signal primarily associated with the stromal compartment, with marked differences in signal intensity and continuity across processing methods. (D–F) Central cornea, showing background or negative staining across all processing methods. (G–I) Kidney tissue, serving as a TAP1-positive control, showing robust TAP1 expression with clear cellular and subcellular definition in Wax and Wax AR sections and reduced structural resolution in cryo-embedded tissue. * marks one positive cell in stroma

Article Snippet: Slides were then incubated with monoclonal primary antibodies Rb P40 (Abcam AB203826, 1:200), Rb Cd68 (Abcam AB213363, 1:100), Rb Pax6 (Abcam AB195045, 1:500), Ms Pax6 (Abcam AB78545, 1:100), Ms CD11c (Abcam AB254183, 1:250), Ms Actin (DSHB AB_528068, 1:8.5), Ms Pax6-S (DSHB AB_528427, 1:12), and Ms TAP1 (DSHB AB_531780, 1:55).

Techniques: Negative Staining, Positive Control, Expressing

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Machine learning-based screening of candidate genes for DKD. ( A-B ) LASSO regression analysis identified 5 candidate genes. ( C-D ) Random forest analysis selected 10 top-ranked genes based on relative importance. ( E ) Venn diagram of the overlapping hub genes (CASP1, PRKX, TAP1) derived from LASSO regression and random forest analysis. (F) Protein-protein interaction network of hub genes generated by GeneMANIA

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Derivative Assay, Generated

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Validation of hub gene (CASP1, PRKX, TAP1) expression patterns in DKD. ( A ) Scatter plots comparing expression levels between DKD patients and controls in the GSE30122 dataset. ( B ) Heatmap of normalized expression for the three hub genes across samples. ( C-E ) Independent validation in renal tubule tissues using Nephroseq v5 database: ( C ) CASP1, ( D ) PRKX, and ( E ) TAP1. ( F-H ) Correlation of hub gene expression with glomerular filtration rate: ( F ) CASP1, ( G ) PRKX, and ( H ) TAP1. Statistical analysis: t-tests for normally distributed data (Fig. 6A, C) and Wilcoxon rank-sum tests for non-normal data (Fig. 6D, E). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, no significance

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Biomarker Discovery, Expressing, Gene Expression, Filtration

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: GSEA of hub genes using GO and KEGG gene sets from MSigDB datasets. ( A-B ) GSEA results for CASP1: ( A ) GO and ( B ) KEGG analyses. ( C-D ) GSEA results for PRKX: ( C ) GO and ( D ) KEGG analyses. ( E-F ) GSEA results for TAP1: ( E ) GO and ( F ) KEGG analyses

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques:

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Immune infiltration analysis in DKD. ( A ) Box plot showing the relative abundance of 22 immune cell types across different groups. ( B-D ) correlation analysis between the expression of hub genes and infiltrating immune cell proportions: ( B ) PRKX, ( C ) TAP1, and ( D ) CASP1. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, no significance

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Expressing

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Single-cell RNA sequencing profile of kidney tissues. ( A ) UMAP projection after Harmony batch correction, integrating datasets across samples. ( B ) 22 distinct clusters visualized by UMAP plotting. ( C ) Annotation of 10 major cell types based on marker genes. ( D ) Dot plot illustrating the expression of marker genes in different cell types. ( E-G ) Bubble plots comparing expression patterns of three hub genes in PT cells (DKD vs. controls). ( E ) CASP1, ( F ) TAP1, and ( G ) PRKX. Statistical significance was determined by Wilcoxon rank-sum tests (**** P < 0.0001). LOH, loop of Henle cells; PT, proximal tubule cells; DCT, distal convoluted tubule cells; ENDO, endothelial cells; FIB, fibroblasts; LEUK, leukocytes; ICA, type A intercalated cells; PODO, podocytes; PEC, parietal epithelial cells; ICB, type B intercalated cells

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Single Cell, RNA Sequencing, Marker, Expressing

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Validation of hub genes in experimental and clinical DKD. ( A ) qRT-PCR analysis of TAP1, CASP1, and PRKX expression in HK-2 cells under low glucose (LG, 5.5 mmol/L glucose) or high glucose (HG, 25 mmol/L glucose) conditions. ( B ) Western blot analysis of TAP1 protein levels in LG or HG. ( C ) Representative kidney histology (H&E, PAS, and Masson trichrome staining) in db/m and db/db mice. Scale bar, 20 μm. ( D ) Western blot of TAP1 protein levels in kidney tissues from db/m and db/db mice ( n = 3 for each group). ( E ) Representative IHC staining of TAP1 in db/m ( n = 3) and db/db ( n = 3) mouse kidney tissue. Scale bar, 50 μm. ( F ) Representative IHC staining of TAP1 in human kidney tissues from DKD ( n = 3) and control ( n = 3) patients. Scale bar, 50 μm. * P < 0.05, ** P < 0.01; ns, no significance

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Western Blot, Staining, Immunohistochemistry, Control